Composite

Part:BBa_K4803108:Design

Designed by: Kotaro MURAI   Group: iGEM23_UTokyo   (2023-10-10)


CD4signal-FKBP-CD28TMD-TEVp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 475
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 925
    Illegal SapI.rc site found at 1273


Design Notes

Major restriction enzyme recognition sequences (EcoRI XbaI SpeI PstI NotI BsaⅠ) are removed from this part.

Source

This biobrick is MESA Part created by combining Part:BBa_K4803005(CD4 signal), Part:BBa_K4803004(FKBP), Part:BBa_K4803006(CD28 Transmembrane domain), Part:BBa_K2969000(TEV protease).